Isolation and characterization of endophytic actinomycetes. Journal of chemical and pharmaceutical research, 2017, 910. Pdf actinomycetes endophytes are promosing biological control agents for use in agriculture and have been isolated from various plant species. Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria bacillus cereus. The endophytic actinomycetes which are associated with plants also play important role in protection their host from phytopathogenic invasions 14. Screening of locally isolated actinomycetes and endophytic. Actinoallolides ae, new antitrypanosomal macrolides. Studies on endophytic actinomycetes i streptomyces sp.
In this study, 37 endophytic actinomycetes were isolated from roots of 14. Isolation, abundance and phylogenetic affiliation of. Actinomycetes are one of the most attractive sources of antibiotics and other biologically active substances of high commercial value. Members of this genus are aerobic, grampositive, filamentous bacteria which have the. Endophytic actinomycetes were untouched area for microbiologist, but in recent years it was found to be efficient pr oducer of novel antibiotic and lead compounds to develop new medicines. An actinomycete strain, designated 83ehsut, was isolated from surfacesterilised roots of suaeda maritima, collected from petchburi province, thailand. Full text is available as a scanned copy of the original print version. In the search of novel source of actinomycetes, endophytes have been demonstrated to improve and plant pathogens and various environmental stresses. When the plant is attacked by plant pathogens, the diversity and community structure of plantassociated microbes might be changed. Antimicrobial biosynthetic potential and genetic diversity of. Pdf the diversity, plant growth promoting and anti. Antimicrobial biosynthetic potential and genetic diversity. Endophytic fungi residing in medicinal plants have the.
Thus, we analyzed the antimicrobial potential of 10 actinomycetes isolated from the medicinal plant vochysia divergens located in the pantanal sulmatogrossense, an unexplored wetland in. Isolation and cultivation methods of actinobacteria. Isolation, identification and screening of endophytic bacteria antagonistic to biofilm formers hira muzzamal, rabbia sarwar, imran sajid and shahida hasnain department of microbiology and molecular genetics, university of the punjab, quaideazam campus, lahore 54590, pakistan abstract. Identification of endophytic actinomycetes from indonesian rice plant based on 16s rrna and nifh genes analyses.
Of the 330 isolates recovered, 212 were from roots, 97 from leaves and 21 isolates from stems with a prevalence of 3. A total of 101 endophytic actinobacteria strains were recovered by culturebased. Bioefficacy of endophytic actinomycetes for plant growth. Endophytic actinomycetes refer to actinomycetes that reside in the inner tissues of healthy host plants. Isolation of endophytic actinomycetes from selected plants. Isolation, identification and screening of endophytic. Vijay c verma 0 1 swechha anand 1 christian ulrichs 2 santosh k singh 1 0 department of microbiology, institute of medical sciences, banaras hindu university, varanasi 221005, india 1 centre of experimental medicine and surgery, institute of medical.
Waksman and his students 19431951 which culminated in the discovery of streptomycin and other new and potentially useful chemotherapeutic agents. A novel endophytic actinomycete strain gku 173t isolated from the roots of acacia mangium willd. Links to pubmed are also available for selected references. S5 with herbicidal activity from tomato roots volume 3 issue 1 qiu zhi.
Potatoes solanum tuberosum are not only a regionally relevant food item for residents. Screening of locally isolated actinomycetes and endophytic fungi for production of bioactive compounds in accordance with universiti pertanian malaysia higher degree act 1980 and universiti pertanian malaysia higher degree regulations 1981. Isolation of endophytic streptomyces strains from surface. Endophytic actinomycetes as emerging source for therapeutic. Environmental issues genetic aspects ammonia dna genes genetic research genomics microbiological research phylogeny plant genetics diseases and pests rna sequencing. Isolation of endophytic actinomycetes from roots and leaves of. Actinoallolide a showed the most potent and selective in vitro anti. Taxonomic position of strain 83ehsut was studied using a polyphasic approach. Microorganisms related to plant roots are vital for plant growth and health and considered to be the second genome of the plant. The early exploratory studies by mccormack 1935 and alexopoulos and herrick 19381942 were followed by the intensive studies by professor s. However, a systematic investigation into the abundance and diversity of cultivated actinomycetes residing in tea plants has not been.
Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves. Opredelitel aktinomitsetov identification manual of actinomycetes. Screening of endophytic actinomycetes from different indigenous medicinal plants. The goal of this study is to characterize differences in rootassociated endophytic actinobacterial community composition. The distribution of actinomycetes in various natural habitats, including soil, ocean, extreme environments, plant, lichens and animals, is described.
Investigation of bioactive compounds from endophytic actinomycete submitted by asawin wanbanjob as a partial fulfillment of the requirements for the degree of master of science, program of organic chemistry. A novel source of potential acyl homoserine lactone degrading enzymes surang chankhamhaengdecha,1,2,3 suphatra hongvijit,2,3,4,5 akkaraphol srichaisupakit,1,2,3 pattra charnchai,2,3,4 and watanalai panbangred2,3,4,5 1 department of biology, faculty of science, mahidol university, rama vi road, bangkok 10400, thailand. In vitro and in vivo plant growth promoting activities and dna fingerprinting of antagonistic endophytic actinomycetes associates with medicinal plants. The actinomycetes encompass two different groups of filamentous bacteria. Endophytic actinomycetes are a promising source of novel. Historically, the actinomycetes were called the ray fungi and were thought to be related to the true fungi, such as bread molds, because they formed mats mycelia of branching filaments hyphae. The collection and pretreatment of test samples from different sources, design. Positive strains for antimicrobial testing were then subjected to carbon source utilization testing and viewed under microscope to determine their spores. Insecticidal activity of endophytic actinomycetes isolated. Biogenic gold nanotriangles from saccharomonospora sp. This is a pdf file of an unedited manuscript that has been accepted for publication. Diversity of endophytic actinomycetes from wheat and its. Antibacterial activity of endophytic actinomycetes.
Endophytic actinomycetes international journal of current. A total of 36 endophytic actinomycetes were isolated from roots, stems and leaves of emblica officinalis gaertn gooseberry and identified as streptomyces sp. The structures of the actinoallolides, including absolute stereochemistry, were elucidated by a combination of spectroscopic analyses and a series of chemical derivatization studies. Actinomycete, order actinomycetales, any member of a heterogeneous group of grampositive, generally anaerobic bacteria noted for a filamentous and branching growth pattern that results, in most forms, in an extensive colony, or mycelium. Get a printable copy pdf file of the complete article. The present study was conducted with a view to screen endophytic actinomycetes from four vegetable crops solanum lycopersicum, capsicum annum, solanum tuberosum and cucumis melo and evaluate their potential to produce plant growth promoting and biocontrol agents.
As evident from the recent literature, endophytic actinomycetes are being increasingly explored for new therapeutic molecules which may effectively address the current human health. Antagonistic effect of endophytic actinomycetes was in agreement with the results of identification of endophytic actinomycetes from wheat roots from a range of sites across south australia coombs and franco, 2003 and tomato roots in south china cao, et al. Tea plants camellia sinensis produce arsenals of phytochemicals, which are linked. Meanwhile, streptomyces strain 3bqp1 produces bioactive.
Eltarabily ka, hardy gestj, sivasithamparam k 2010 performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of pythium aphanidermatum, a pathogen of cucumber under commercial field production. Tea plants camellia sinensis produce arsenals of phytochemicals, which are linked to a number of medicinal and nutritional properties. Antibacterial effect of endophytic actinomycetes from marine. The mycelium in some species may break apart to form rod. Endophytic actinomycetes were isolated from 6 different plants collected from uttarakhand, india. This chapter focuses on the potential of endophytic actinomycetes as a biocontrol tools of plant diseases and b sources of plant growth promoters, as well as. Diversity, novelty, and antimicrobial activity of endophytic. Isolation of endophytic actinomycetes from roots and leaves. Vijay c verma 0 1 swechha anand 1 christian ulrichs 2 santosh k singh 1 0 department of microbiology, institute of medical sciences, banaras hindu university, varanasi 221005, india 1 centre of. Endophytic actinomycetes from medicinal plants produce a wide diversity of secondary metabolites sm. To our knowledge, this is a first report on genetic diversity, antimicrobial evaluation and biosynthetic genes of endophytic actinomycetes associated with these medicinal plants of gibbon wild life sanctuary, assam. The goal of this study is to characterize differences in rootassociated endophytic actinobacterial community composition and.
Environmental issues genetic aspects ammonia dna genes genetic research genomics microbiological research phylogeny plant genetics diseases and pests rna sequencing research. The genus micromonospora was firstly proposed in 1923 by orskov and classified as the type genus of the family micromonosporaceae within the phylum actinobacteria. Actinomycetes isolates were screened for their herbicidal activity against parthenium hysterophorus, ageratum conyzoides and bidens biternata. Get a printable copy pdf file of the complete article 715k, or click on a page image below to browse page by page. An actinomycete, designated strain yim 564t, was isolated from the root of a chinese medicinal plant, carex baccans nees, collected from yunnan, southwest china, and subjected to a polyphasic taxonomic study. Actinobacteria have high guanine and cytosine content in their dna. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. But, no such information was found in the literature regarding the bioherbicidal effect of endophytic actinomycetes against the weed plants of ageratum conyzoides, bidens biternata and parthenium hysterophorus. Endophytic strains belonging to this group have been isolated from a wide range of plants, including crop plants, medicinal plants, halophytes, and even some woody tree species 7 15.
Endophytes produce a number of compounds in order to maintain the symbiotic association with plants, to promote plants growth and help them to survive in the plant environment. Insecticidal activity of endophytic actinomycetes isolated from azadirachta indica against myzus persicae. Full text full text is available as a scanned copy of the original print version. The committee recommends that candidate be awarded the relevant degree. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. A novel source of potential acyl homoserine lactone degrading enzymes surang chankhamhaengdecha,1,2,3 suphatra hongvijit,2,3,4,5 akkaraphol srichaisupakit,1,2,3 pattra charnchai,2,3,4 and watanalai panbangred2,3,4,5 1 department of biology, faculty of science, mahidol university, rama vi. A novel antibiotic source ravi ranjan kumar1 and vasantba j. Isolation of endophytic actinomycetes from roots and leaves of maize zea mays. In the search of novel source of actinomycetes, endophytes have been demonstrated to improve and promote growth of host plants as well as to. Each of tlc fraction was able to inhibit growth of b. Totally 6 endophytic actinomycetes were obtained from all the samples. A total of 35 endophytic actinomycetes strains was isolated from the roots, stems and leaves tissues of.
At the time of writing, the genus micromonospora consisted of 84 species and 7 subspecies with validly published names. Isolation and characterization of endophytic streptomyces sp. Screening of endophytic actinomycetes from different. Actinobacteria actinomycetes have been received much attention, as these bacteria produce a variety of natural drugs and other bioactive metabolites.
Several endophytic actinomycetes act as plant growth promoter by producing of phytohormone, indole3 acetic acid iaa or iron chelating molecules, siderophores in vitro 15. However, to date, the knowledge about endophytes from brazil remains scarce. Lorneic acid analogues from an endophytic actinomycete. To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of rhododendron were surfacesterilized for use as an isolation source. Identification of endophytic actinomycetes was based on their morphology and the. Our natural products discovery program utilizes endophytic actinomycetes associated with plants and employs biological assays and hplcbased metabolite profiles as the preliminary screen to identify strains of interest, followed by largescale fermentation and isolation, leading to new andor bioactive natural products. Six new trialkylsubstituted aromatic acids, namely, lorneic acids ej. Isolation of actinomycetes were done using humic acid bvitamins agar hva while endophytic fungi were from potato dextrose agar pda. Isolation of endophytic actinomycetes from roots and. The isolation of endophytic actinomycetes from surfacesterilized tissues of 36 plant species was made using humic acidvitamin hv agar as a selection medium. Virani science collage, kalawad road, rajkot, gujarat360005 2department of microbiology, shree m. Species distribution and biological diversity of endophytic actinobacteria of medicinal plants are extensively influenced by ecological environment hou et al. These isolates belong to the seven different genera. Isolation and characterization of endophytic streptomyces.
In this study, we have investigated the diversity of endophytic actinomycetes associated with the six traditional medicinal plants. Actinomycetes article about actinomycetes by the free. Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that various species. Endophytic actinomycetes associated with plant roots are a relatively untapped source of potential new bioactive compounds. It varied from other actinomycetes by utilize the nutrients, sugar moieties, carbohydrate polymers, amino acids and peptides from. International nano letters biogenic gold nanotriangles from saccharomonospora sp. If the inline pdf is not rendering correctly, you can download the pdf file here. An analysis of 16s rrna gene sequence similarities showed that strain yim 564t was a member of the genus glycomyces, being most closely related to. Endophytic actinomycetes to enhance sustainable agricultural productivity endophytic actinomycetes to enhance sustainable agricultural productivity franco, christopher 20081001 00. Screening of endophytic actinomycetes for their herbicidal. Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts. In fact, it was the only characteristic which was used in many early descriptions, particularly of streptomyces species in the first few editions of bergeys manual.
Microorganisms free fulltext community structures and. Pdf isolation and identification of endophytic actinomycetes and. Eleven actinobacterial strains were isolated from different plants, lentil lens esculentus, chickpea cicer arietinum l. Phylogenetic analysis, based on 16s rrna gene, indicated that strain gku 173t was the most closely related to fodinicola feengrottensis hki 0501tthe only species in the genus fodinicola. Performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the united arab emirates. Antibacterial activity of endophytic actinomycetes isolated. Therefore, bioherbicidal effect of endophytic actinomycetes against these weeds was performed in preemergence and postemergence.
Attractive biocontrol agents, journal of general plant pathology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Characteristics of soil actinomycetes from antarctica penka moncheva1, sava tishkov1, nadezhda dimitrova1, valentina chipeva2, stefka antonovanikolova1 and nevena bogatzevska3 1sofia university, biological faculty, department of general and industrial microbiology. Diversity and antimicrobial activity of endophytic actinomycetes. In the present study, 85 strains of actinomycetes were isolated from the neem tree azadirachta indica and screened for their insecticidal activity against the green peach aphid, myzus persicae. Actinobacteriaderived antibiotics that are important in medicine include aminoglycosides, anthracyclines, chloramphenicol, macrolide, tetracyclines, etc. Journal of chemical and pharmaceutical research, 2017, 9. Isolation and cultivation methods of actinobacteria intechopen. Five new antitrypanosomal macrolides, actinoallolides ae 15, were discovered from the cultured broth of actinoallomurus fulvus mk10036. Isolation of endophytic actinomycetes from catharanthes roseus l. Biocontrol of chickpea root rot using endophytic actinobacteria. Endophytic actinomycetes from tea plants camellia sinensis. Endophytic actinomycetes as potential agents to control.
Isolation of endophytic streptomyces strains from surfacesterilized roots. Isolated strains were then subjected to enzymatic and also antimicrobial testing. Isolates were characterized and identified morphologically as well as using 16s ribosomal rna gene sequencing. Source, identification, and their applications mukesh sharma, pinki dangi and meenakshi choudhary department of biotechnology, jaipur institute of biotechnology, maharaj vinayak global university, jaipur rajasthan india corresponding author a b s t r a c t production of primary and secondary metabolites int. Antibacterial effect of endophytic actinomycetes from. Endophytic actinomycetes are a promising source of novel metabolites with diverse biological activities. The collection and pretreatment of test samples from different sources, design principle of. Isolation of actinomycetes from soil stephen kugbere agadagba department of biochemistry, faculty of life sciences, university of benin, benin city, nigeria abstract the main focus of this study was to isolate some antibiotic producing actinomycetes strains from soil. Pdf screening of endophytic actinomycetes for their. Identification of endophytic actinomycetes from indonesian. Thus, we analyzed the antimicrobial potential of 10 actinomycetes isolated from the medicinal plant vochysia divergens located in the pantanal sulmatogrossense, an unexplored wetland in brazil. Phylogenetic determination based on the 16s rrna gene sequence similarity showed that strain 83ehsut belongs to the genus. Frontiers diversity and applications of endophytic.
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